Wednesday, August 15, 2007

Colorimetric Micorarray detection should be popular


Colorimetric detection of microarrays should be more popular than it is currently. It is prefect for all DNA and protein microarray applications and is 1/10th the cost of fluorescent based approaches in both reagent cost and equipment. Assays can be more sensitive than fluorescent assays, since the enzymatic amplification of signal is easy to achieve in colorimetric assays and the assays are miniaturized to the same level as fluorescence. Membrane slides such as nylon and nitrocellulose used in colorimetric assays do not cost more than glass. For detection we've made what we call the SpotWare microarray scanner. It provides the ability to change the gain on the detector, thus making very faint signals that cannot be seen by eye under a microscope, very easy to quantitate....thus making the system very sensitive. SpotWare provides histogram feedback in the software interface to help the end user determine the appropriate gain setting to provide the best signal to noise ratio and preserve quantitative data for the images acquired. The deck of the scanner sets the slides in the proper orientation, and the interface makes selecting image acquisition areas using a mouse easy to do. Pre-scans can be done to zoom in on target areas that contain spots, thus making it easy to find spots and keep image sizes as small as possible. Resolution is easily changed between 5, 10, 20 and 50 microns. 12 slides can be oriented on the deck, with the slide to be scanned selected in software. SpotWare scanner creates 16-bit gray scale tif images, industry standard for microarray quantitation. The tiff image format allows multiple images to be saved in a single scanned file and we've setup SpotWare to save 3 files within the single saved tiff file..., the actual 16-bit data, the barcode area for identification, and the pre-scan image. This system is prefect for all DNA and protein microarray applications and is 1/10th the cost of fluorescent microarray scanners. Assays can be more sensitive than fluorescent assays, since the enzymatic amplification of signal is easy to achieve in colorimetric assays...see arrayit.com for more information.
http://www.arrayit.com/Products/Microarray_Scanners/microarray_scanners.html