Tuesday, September 1, 2009

Peptide Microarrays

The data above was generated shows that the FLAG peptide epitopes were recognized by the ANTI-FLAG antibodies, but not by the ANTI-CD28 antibody used as an isotype matched control (negative control). In addition, the scrambled FLAG sequence that was used as another negative control was also not recognized by the ANTI-FLAG antibodies. These results indicate the specificity of the peptide-antibody binding. The data also indicate that peptides containing additional sequences from both PILL and GST demonstrated enhanced reactivity with the ANTI-FLAG antibody. Adding PILL or GST sequences to the peptides did not enhance reactivity to the irrelevant antibody. Data courtesy of Dr. Jonathan Graf and Dr. John Imboden, UCSF. Peptides courtesy of Sigma-Aldrich.
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