Wednesday, January 27, 2010

Microarray Immunoassay Experimental Design

Arrayit's microarray platform is highly flexible and powerful suite of technologies for miniaturizing and multiplexing existing assays such as those for gene expression, genotyping, immunoassays and others.
Microarray immunoassay test development requires a little work up front, but it's fun to do. Microarrays are highly affordable and can reduce the cost of running immunoassays, we welcome the opportunity to manufacture custom microarrays to meet almost any experimental need. Here is a list of questions to help guide the process of experimental design. You don't have to answer all these questions to have a legitimate project, questions are not applicable to all projects. We sign confidentiality agreements, we don't expect customers to disclose things they do not wish to disclose! Contact me at todd@arrayit.com, 408-744-1331 with any questions.
  1. How will the microarray assay be used and what are the goals of the experiments or tests?
  2. What is the specific biomolecule or analyte that you want to measure?
  3. Have ELISA assays been run with the sample types?
  4. What capture analytes are we printing into a microarray?
  5. What are the characteristics of the capture analytes for the microarray?
  6. Antigen, antibody, cell lysate, peptide...other?
  7. If antibodies, do you have a secondary antibody for each capture antibody?
  8. Amount of purified capture analyte available?
  9. If the analytes are peptides do you have all the amino acid sequences?
  10. Are there any safety issues associated with the handling or use of the analytes?
  11. Storage buffer (including pH)?
  12. Soluble in water or is another buffer required?
  13. Storage conditions?
  14. If analyte is lyophilized, were any stabilizers used?
  15. What is the origin of the sample to be tested...blood, serum, plasma, water, tissue culture supernatants...?
  16. Do the samples to be tested on the microarray have to be pre-treated, such as extractions or centrifugations? If yes, what are they?
  17. What is the expected outcome of the immunoassay you want to develop?
  18. Are we developing a microarray assay that will require routine testing?
  19. How many samples will be tested overall?
  20. How many samples do you want to test for at one time?
  21. Do you require automation, or is manual processing OK?
  22. Did you want to outsource the testing or do parts or all of it?
  23. Do you want to be empowered to make your own microarrays, or have us do it for you? (costs less if we do it).
  24. Do you have a microarray scanner or other instrument for reading the test?
  25. Are you planning fluorescent, colorimetric, chemiluminescent, SPR or other detection platform?
  26. Does your test require FDA approval (or will it in the future)?
  27. Is there anything else important you think we should know?

http://www.arrayit.com/Services/Protein_Microarrays/protein_microarrays.html