Wednesday, February 16, 2011

Protein & Peptide Microarrays

Tools for understanding the interaction of biomolecules...Arrayit microarrays enable the miniaturized, mutiplexed and parallel testing of many proteins and peptides in a single assay. Unlike bead formats which suffer from steric hinderence between beads, cross reactivity and poor shelf life, biomolecules printed into microararys can only react with the solutions that are applied to them, provide specific binding and have a extremely long shelf life. Capture biomolecules are spotted and stabilized onto a glass substrate in sub-nanoliter volumes and can be run in a 96 well format using standad ELISA automation and scanned on a standard microarray scanner. These capture biomolecules on the microaray can be peptides, proteins, antibodies, or carbohydrates and are used to bind to biomolecules present in solution applied to the microarray. Detection and quantification is done with fluorescence, colorimetric and chemiluminescent methods, with fluorescence being the most popluar due to it’s superior sensitivty and quantitative performance. Peptide and protein microarrays offer many applications such as miniaturizing and multiplexing existing ELISA assays, the detection of infections, study of autoimmune diseases, epitope mapping, simultaneous profiling of proteins, biomarker discovery and more. Click here for more information.