Monday, June 11, 2012

soil microbe test

The DIM-Array Nitrogen Cycle system is a DNA microarray based test that measures and reports the effect of agricultural management practices, fertilizer treatments and environmental factors on the quantity and proportion of bacteria involved in key processes of the nitrogen cycle (nitrogen fixation, nitrification, and denitrification) in the rhizosphere (the narrow region of soil that is directly influenced by root secretions and associated soil microorganisms).
 Since nitrogen is a key nutrient determining the productivity of agroecosystems, it is of central importance to optimize the nitrogen balance, mainly by steering genetic the resources of soil microbes in a way that losses of applied nitrogen are minimized and biological nitrogen fixation is increased.
Plant and grain yields might benefit from reduced nitrogen losses by denitrification and enhanced biological nitrogen fixation.

Custom microarrays are also available.  The microarray and assays, designed by Dimole and manufactured by Arrayit for environmental testing, work on a PCR-based microarray molecular technique that enables rapid and simultaneous detection of multiple organisms (viruses, bacteria, fungi, yeasts, and others). In addition, the assay quantifies each organism on a rating scale to guide intervention methods. An Internal Amplification Control is integrated into the system in order to avoid false-negative results. Microarrays allow for greater timeliness and efficiency in receiving results, while significantly reducing cost per sample.  The microarray platform enables the a more desirable testing system than the traditional enzyme linked immunosorbent assay (ELISA) or polymerase chain reaction (PCR).
In particular:
They allow for the detection of several diseases in one test, in comparison to ELISA and PCR which allow for testing of only one disease at a time.
They allow for testing of dormant viruses, eliminating the need for costly grow-up of sample.
They are useful to identify secondary diseases that will result in the symptom expression of the primary infection.
 They can be used for testing of multiple types of disease-causing agents, including bacteria, viruses, viroids, fungi and nematodes.
They are quantitative.
They enable testing of DNA from a large variety of substrates.
Antibodies used for ELISA generally do not allow for detection down to strain-specific levels, however, microarrays, like PCR, allow for specific detection of DNA sequences.
Contact:
www.dimole.ca  and www.arrayit.com contact@dimole.com, todd@arrayit.com for more information.