Friday, September 28, 2012

Protein Microarray Experimental Design

We get you from Pilot Study to Production.

We're getting requests for protein microarray manufacturing.  There are several detection methods and formats to choose from.  There are also different regulatory requirements.  We have our proven methods in place to guide any project if you are not sure what you want or how to accomplish your goals.  Both the scientific and business needs must be met.  Making the science and business match is our job.  The list of questions below gets us started to developing a budget and getting a project going on a solid scientific foundation built on the Arrayit Platform...from prototype, to pilot study and into production.

Arrayit's microarray platform is highly flexible and powerful suite of technologies for miniaturizing and multiplexing existing assays such as those for gene expression, genotyping, immunoassays and others.  Protein Microarray test development requires a little work up front, but it's fun to do. Here is a list of questions to help guide the process of experimental design.  You don't have to answer all these questions to have a legitimate project, questions are not applicable to all projects.  We sign confidentiality agreements, we don't expect customers to disclose things they do not wish to disclose! Contact: or phone 408-744-1331     
  • How will the microarray assay be used and what are the goals of the experiments or tests?
  • What is the specific biomolecule or analyte that you want to measure?
  • Have ELISA assays been run with the sample types?
  • What capture agents are we printing into a microarray?
  • What are the characteristics of the capture agents on the microarray?
  • Antigen, antibody, cell lysate, peptide...other?
  • If antibodies are the capture agents, do you have antibody pairs?
  • Amount of purified capture agent available?
  • If the capture agents are peptides do you have all the amino acid sequences?
  • Are there any general toxicity or safety issues associated with the handling or use of the capture agents?
  • Storage buffer (including pH)?
  • Soluble in water or is another buffer required?
  • Storage conditions?
  • If the capture agents are lyophilized, were stabilizers used?
  • What is the origin of the sample to be tested...blood, serum, plasma, water, tissue culture supernatants, etc.)?
  • Do the samples to be tested on the microarray have to be pre-treated, such as extractions or centrifugations? If yes, what are they?
  • What is the expected outcome of the assay you want to develop?
  • Are we developing an assay that will require routine testing?
  • How many samples will be tested overall?
  • How many samples do you want to test for at one time?
  • Do you require automation, or is manual processing OK?
  • Did you want to outsource the testing or do parts or all of it?
  • Do you have a microarray scanner or other instrument for reading the test?
  • Are you planning fluorescent, colorimetric, chemiluminescent, SPR or other detection platform?
  • Does your test require FDA approval (or will it in the future)?
  • Is there anything else important you think we should know?
Kind regards,
Todd Martinsky