Friday, June 12, 2015

Complete Cellular Process Analysis using Microarray Technology.

None of our competitors can put whole cells into a microarray format, but Arrayit can. Complete understanding of cellular processes is the holy grail of diagnostics and therapeutics, because everything else is measuring components of cells (proteins, DNA, carbohydrates {other types of arrays we also make}) and you can't get the whole story reading only 1 chapter. Every part of the story is critical, but the ending is the payoff. In this new peer reviewed publication titled, "Multiscattering-enhanced optical biosensor: multiplexed, non-invasive and continuous measurements of cellular processes" published by the Swiss Federal Institute of Technology, we see Arrayit technology being implemented to analyze complete cellular processes. Cell based microarrays is a application poised for massive growth we're proud to be playing an important role in this wonderful science.  This paper is especially interesting because not only does it combine cellular analysis and microarray, but also combines micro-fluidics. 

Multiscattering-enhanced optical biosensor: multiplexed, non-invasive and continuous measurements of cellular processes

Volodymyr B. Koman, Christian Santschi, and Olivier J. F. Martin


The continuous measurement of uptake or release of biomarkers provides invaluable information for understanding and monitoring the metabolism of cells. In this work, a multiscattering-enhanced optical biosensor for the multiplexed, non-invasive, and continuous detection of hydrogen peroxide (H2O2), lactate and glucose is presented. The sensing scheme is based on optical monitoring of the oxidation state of the metalloprotein cytochrome c (cyt c). The analyte of interest is enzymatically converted into H2O2 leading to an oxidation of the cyt c. Contact microspotting is used to prepare nanoliter-sized sensing spots containing either pure cyt c, a mixture of cyt c with glucose oxidase (GOx) to detect glucose, or a mixture of cyt c with lactate oxidase (LOx) to detect lactate. The sensing spots are embedded in a multiscattering porous medium that enhances the optical signal. We achieve limits of detection down to 240 nM and 110 nM for lactate and glucose, respectively. A microfluidic embodiment enables multiplexed and crosstalk-free experiments on living organisms. As an example, we study the uptake of exogenously supplied glucose by the green algae Chlamydomonas reinhardtii and simultaneously monitor the stress-related generation of H2O2. This multifunctional detection scheme provides a powerful tool to study biochemical processes at cellular level.