Thursday, March 24, 2016

High Throughput Blood Testing

How we run the equivalent of 10,368 blood based ELISA assays on only 4 microscope slide size pieces of glass in a single tool with the footprint of a standard 96 well plate using blood input levels from fingerstick collection. I realize this looks complicated and perhaps even sounds impossible to you. But it's all quite simple really and it works. Tracking the data generated on our miniaturized multiplexed and parallelized microarray testing platform is critical. The first photo is the form we use in the lab for our AHC4x12 hybridization cassette (also pictured). The slide numbers, block numbers for microarray quantification, and blood sample numbers are all fixed based on the cassette hardware and the microarray manufacturing pattern which are displayed on the form. Each blood sample is reacted to 2 microarray "blocks" of spots for up to 48 samples per tool (see 2 photos of single blocks here). We write on this form the slide number, date, microarray type, and bar code number from the blood sample that is applied to the microarray. It works and tracks everything perfect. Total test time only 4 hours all the way through to final data reports, 1 technician, total sample preparation and applying the sample to the microarrays requires only 144 pipetting steps for 10,368 data points.
Form Used in the lab to track blood samples
AHC4x12 Hybridization Cassette.  Each "well" is a microarray of 2 blocks of spots.
 Example block 1
Example Block 2
(16-bit raw image data presented in pseudo color scale)