Friday, April 15, 2016

Whole Blood Collection Card for Protein Microarray

Whole blood collection cards to acquire and stabilize, RNA, DNA and Protein from lancet "finger-stick" collection methods are important tools in life sciences and healthcare.  The best and most sensitive assays require sample input of the highest integrity.  The old saying is especially true in the laboratory, "Garage in garbage out".  The sample input integrity is essential to acquiring great results.

The most important aspect of any dry blood spot collection card is the purity of the collection matrix that the blood sample is applied.  There can be no contamination on the matrix that will compromise the sample being collected. All material used to manufacture the collection matrix should use the highest purity materials validated by chromatography and use high purity deionized water and chemicals in the process. The final collection matrix holding the whole blood must be free of naturally occurring RNases, DNases, and Proteases, the enzymes that break down and degrade these important biomolecules that exist everywhere! Therefore, the cards should be assembled in clean room environments free of contamination.  Each collection matrix must be homogenous in composition.  Uniformity, thickness, and absorbency characteristics must be consistent across all cards.  

The collection matrix used for Arrayit Whole Blood Collection Cards are enclosed in a convenient cover with a flap that easily opens and closes to protect the blood spot collection area from environmental contamination or damage.  Each is bar coded for unique identification and has space on the front of the card for information the specimen collection personnel may want write on the card.  Logos and text can all be customized to meet customers specific requirements. Cards can come in bulk or packed individually in environmental proof bags.

For use in protein microarray, cards come with specially formulated elution/reaction buffers with spin columns to prepare the sample to be applied to protein microarrays.  Here is a training video of the process of elution.

In general, a single 1/2 inch circle is enough for a single robust protein microarray reaction.  There is no need to separate serum and internal Arrayit studies have shown that whole blood eluted from the card in our reaction buffer can provide superior results to wet serum alone in specific applications. The process of separating serum from whole blood can selectively eliminate or reduce the concentration of certain proteins and other biomolecules that may be desirable to measure on a microarray.  One 1/2 circle of eluate mixed with the reaction buffer provided with the kit yields a 120 microliter reaction cocktail ready to apply to a microarray.  For larger microarrays, more circles may be used.  For lower volume microarray reactions, partial use of the cocktail may be applied to the microarray. For best results use all complimentary microarray surface chemistry, buffers, and solutions.  Traditional microarray and ELISA buffers are not suitable for proper elution and reaction.  Contact me for more details,